The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. Temperature control and shaking come as standard. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. . 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Cell Cytotoxicity assay procedure. 20 μL of Component A was added to each well of the microplate. ATP standard curves read on SpectraMax microplate readers. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Molecular Devices. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. e. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. On the back, in the right hand top corner,. Published Dec 16, 2020 | Updated Dec 16, 2020. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. View the Molecular Devices website. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 003 fmol 0. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. . 305 reference level saturation. Products : General, Microplate Readers. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Find regional support. SpectraMax 250 User. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Brochure. Other features and benefits include: Eliminate changing out filters. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. . The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Microplate reader settings. The SpectraMax 190 reader can be integrated with Molecular . The calculated LOQ values were 0. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Reader modes are customized to your specific applications with options to upgrade easily. The calculated LOQ values were 0. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Download PDF. A: Microplate Preparation. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Microplate automation and detection instruments for walk away operation and high quality results. Find regional support. microplate reader. Dual-Glo luciferase assay system (Promega Cat. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. The plate reader can measure multiple readouts, including UV. The University of Rouen in Normandy uses the SpectraMax iD3 and. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Step 4: Incubate at room temperature for 1 hour, protected from light. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. begin reading microplates with seven mouse clicks. 300 µL of Detergent Compatible Bradford reagent was added to each well. Microplate Readers BioTek Epoch 2 Microplate Reader. These robust readers have been placed in labs from. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 100 fmol 1536-well (8 µL) 0. 4 μL DNA standards. Most papers they cultured bacteria to OD600>0. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Endpoint, kinetic, spectrum. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Table 2 summarizes the results for the uniformity test. Download PDF versions of the SpectraMax Microplate Reader User Guides. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). \ Figure 3. Fluorescence Microplate Reader Comparison Chart. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. Lift up the lid over the chamber to insert or remove a cuvette. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. Positions H21-K22 contained plate blanks of PBS only. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). When the user applies a set temperature of 37°C, the top is heated to 37. SAN JOSE, CA — Molecular Devices, LLC. 2 only) SpectraMax M-series Microplate readers. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 020 fmol 0. Measuring platelet aggregation with a microplate reader. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. microplate reader. Example: [Drawer]. For SpectraMax ABS Plus reader, r 2 = 0. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. begin reading microplates with seven mouse clicks. Plate chamber design of the SpectraMax category 1 microplate readers. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The screw remains inside the instrument. 1. Endpoint, kinetic, spectrum. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. In addition, the SpectraMax iD5 can be expanded to. 58% for the detection module and 2. flexibility of monochromator-based systems. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. SpectraMax ® M2 Multi-Mode Microplate Reader. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax 190 reader can be integrated with Molecular . 0 out of 5 $ 5,999. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax Glo Steady-Luc Reporter Assay Kit. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. 6% for the on-board optics (monochromators). User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. . Unlock your i3/i3x instrument after shipment. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Products. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. We would like to show you a description here but the site won’t allow us. 380 nm/Exc. The guide also covers the software features, the specifications, and the accessories of the systems. Fully tested with validations, warranty. Supplier: Molecular Devices. Page 28 Chapter 5: Read Modes and Read Types 1. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Table of Contents. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. Figure 3. edu) to be added as a trained user in iLab. The dual monochromator optics allow. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. B: Selection of Excitation Wavelength. 1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Introduction. The SpectraMax® ABS Plus Microplate Reader can run . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Cell Cytotoxicity assay procedure. Contents of wells were pipetted up and down several times to mix. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Guides: SpectraMax Mini Installation Guide. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Temperature control. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 1. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Unparalleled performance on a personalized platform. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. . The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Step 1: Turn on your microplate reader. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Our specialization servicing. We would like to show you a description here but the site won’t allow us. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. FI assay. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. We would like to show you a description here but the site won’t allow us. M2. Detailed specifications of each model reader are reported in Section. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. For readers without a cuvette port, simply check the box next to ‘PathCheck’. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Step 1: Open the SoftMax Pro IMAP FP protocol. The SpectraMax® iD3 Multi Mode Microplate . com 4. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The plate reader can measure multiple readouts, including UV and visible absorbance. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Absorbance (optical density, OD) was read at 570 nm and 605. keeping with best practices, reader performance should. Total volume (except deep. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. HTRF-compatible SpectraMax Multi-Mode Readers. 6 for further experiment, so I'm confused which i would choose for OD value determination. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Selection of Water Constant in PathCheck settings. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. The 2-channel configuration is available with 0, 2, or 4 injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. 7°C and the bottom to 36. Wide range of applications. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 997 (n = 3). Figure 6: Transcreener ADP2 FI assay principle. Molecular Interactions. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Italic and boldface type are used for emphasis. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. With optimized reagents, option to operate the. Download the SpectraMax Mini Reader Plug-in and associated guides. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. The plate reader optics are tunable for. Download PDF. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Additionally, user-installable options allow the SpectraMax i3 System to. Molecular Devices. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Request a Quote!Introduction. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 76358-624. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. . 1. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. No accessories are needed and SoftMax® Pro Software. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. . SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Selection of Water Constant in PathCheck settings. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Add to Cart. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Examples: “Press carefully to engage,” “ Do not. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. 40266 Email: drugdiscoverytech@lifetech. Microplate Spectrophotometers. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. This unlocks the transport slide. Published Dec 16, 2020 | Updated May 19, 2023. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The CellTiter-Glo assay from Promega uses luciferase. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The advanced optical and. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. western blot membrane detection in a multimode microplate reader platform. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. 7°C and the bottom to 36. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Align the two slots on the bottle holder with the two pegs on the rail. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. In this product video, see how the SpectraMax M5 quickly reads samples. Other technologies such as imaging. Step 2: Launch SoftMax Pro Software. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The advanced optical and. flexibility of monochromator-based systems. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Results are shown in Table 1. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. , April 14, 2022 – Molecular Devices, LLC. Figure 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The guide also covers the software features, the specifications, and the accessories of the systems. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. . . SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Maximum signal with lowest background. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. The SpectraMax M2 and M2e are versatile and reliable. 5 87. 316 grating motor driver faulty. Compact and tunable absorbance microplate readers DATA SHEET. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 100 readings per well gives optimal Z’ values for this assay. Wide range of applications The VersaMax reader covers. . 0 out of 5 $ 23,995 $ 22,495. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 2 only: SpectraMax Mini Update. Prerequisites: SoftMax Pro 7. Our absorbance plate readers feature our PathCheck Sensor technology and. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. For readers without a cuvette port, these options are not shown in the software. SpectraMax Mini User Guide. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. More Product Information. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Category 2 and 3 microplate readers use gradient heating as well. 470 nm). San Jose, Calif. Add to Cart. The screw remains inside the instrument. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. Molecular Devices SpectraMax M2 Multimode Plate Reader. Table 1. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Introduction. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Microplate Readers. The dual monochromator optics allow the. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument.